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Process TMT11 plex data in PD 1 4! ...cause somebody really did ask for it...if youve got TMT11-plex reagent on the way and are using PD 1.4, youll need to add a channel, or download this template from my DropBox. DISCLAIMERS: 1) Tandem Mass Tags are the trademark property of Proteome Sciences. Id put trademarks every time I mention these reagents on the blog, but I dont have access to symbols without using HTML. 2)  Im not a professional mass spectrometrist 3) This looks like it works fine. No promises, though! 4) I totally made up the mass in the average mass box for the method. Youve got to use HRAM to even use the TMT11-plex reagent, so I figure it doesnt matter. I took the difference between the C and N average masses in the box for the other reagents, and added that to the average for the original 131 reagent. No promises. 5) Use at your own risk! download  file  now

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PTMOracle Visualize PTMs !!! in Protein Protein Interaction data!! I dont have proof yet that this is as good as I think it might be. If it is, this is a serious missing link in proteomics filled in (courtesy of free software!!!) What I might be ridiculously excited about is the PTMOracle, which you can read about in this new JPR paper here. If you start doing any kind of PTM study and want to move to downstream analysis you will quickly find you need to: 1) Hijack some genomics tools built years ago for microarrays 2) Use some genomics tools someone hijacked years ago for microarrays and made kinda good at proteomics (and available) 3) Do lots and lots of manual work Those genomics tools are awesome. They really are. On the downstream analysis/data processing end, theyve got a 10 year head start on us. But -- upregulation of the entire ERK protein may do something very different in a cell than ERK phosphorylation on the normal active tyrosine (or the less common, biologically, but eas...

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