NYEPLAK

Proteomic analysis of human cells grown in bacterial cellulose A LOT of biological studies of human cells occurs with cells grown in culture. And we can thank cell culture techniques for a huge amount of what we know of how cells work, but it has been no secret that there are some limitations to cell culture techniques. This is a nice open review on limitations -- with a strong title. Over the last 15 years or so there have been numerous ideas put forward to bridge the gap between cells growing in monolayers in cell culture and cells as they actually exist in human tissues and organs. Growing cells in 3 dimensions is something that has been discussed for years, but -- so far hasnt yielded much in the way of new understandings in biology. Please dont take this as a criticism, I know two great people working hard on this stuff right now who will have stuff out soon, but these complex bioreactors seem to come with their own bevy of complications -- which better technology may soon fix. In...

Ready for a new PTM to worry about Cysteine glycosylation is all over the place in human cells! Fun fact: Did you know that O-GlcNAc modified proteins were discovered in Baltimore over 30 years ago? See, theres more to my home town than fantastic infrastructure and friendly people! Glycoproteomics is kind of exploding right now -- the enrichments are better, the separations are better, and the mass specs are ridiculously better, and the software has almost caught up....and I wonder if this great new paper at ACS is just the tip of the iceberg.... A whole new class of glycopeptides right under our noses! The evidence looks pretty clear cut to me -- and first analysis from this group suggests that it isnt even rare. Once they had a mechanism to enrich and a pipeline to search for them in the data they report proteins with this modification in virtually every subcellular fraction! download  file  now