NYEPLAK

The Manual of Cardiovascular Proteomics My local library finally got this book in a few weeks ago! Im never going to read the whole book. Chapters like "a history of proteomics" and reviews on bottom up and top down proteomics arent meant for me. They are written for cardiovascular researchers who are wondering what this proteomics thing can do for them. What I have read is pertinent to these questions: Why would you want to do cardiovascular proteomics? (the intro and first chapter) and chapter 5 -- Vascular proteomics. Let me start off with stealing this kind of shocking chart from chapter 1 (authors or Springer, please let me know if you want this image taken down (orsburn@vt.edu) This is the number of studies that have been done in this field! Like all of proteomics -- more stuff all the time, but when you consider that a lot of cardiovascular diseases are environmentally driven -- not genetic or driven by mutations -- it is easy to wonder if this is nearly enough work in...

Proteomics postdoc needed in my neighborhood! Want to do super cool and probably super secret proteomics with a power-packed young team in Maryland? Want to make substantially more than I did during my postdocs (maybe more than I did as an NIH staff scientist? I do not want to know if that is actually true...but I suspect it might be...) Check out this posting from my neighbors!  I know they are doing some really cool stuff from what theyve been publishing this year and I suspect from the quality of the team and their instrumentation that this is the tip of the iceberg. download  file  now

Proteomics of mice in space!! Im up stupid early in the morning and have to type this reeeal fast, but Im way to excited to let it wait till Im home after work, cause its Miiiiiiiiice iiiiiiiiiiinnnnn Spaaaaaaaaaaace (sounds like this in my head --warning, audio!) Before I have too much fun with this important study, this is what Im talking about -- in this months JPR. This is important stuff, especially for any of us who grew up under the Asimovian assumptions that humans take the to the stars or our story ends here in our inevitable extinction. Which -- considering that advanced civilizations may receive decades of our television far before our arrival makes it seem less bad now than it did when I was younger. "Hello species that hasnt had war in 1 billion years -- yes...I come from the Tony Danza planet...." It has always been really really hard to become an astronaut/cosmonaut. This was extremely well publicized here --especially with the Apollo program -- those dudes wer...

Proteomics of hydrophobic samples! Need to do some proteomics on something like...adipose tissue...that is mostly lipids?  If trying 27 different lysis/digestion techniques to find the best one doesnt sound like a good time, you should check out this paper where they already did it! A major emphasis of the study is quantitative reproducibility in these tough samples. download  file  now

Proteomics of CSF of children with meningitis Yikes. So...nothing is supposed to be in your cerebral spinal fluid (CSF) except the stuff that is supposed to be in there...and especially not a detectable amount of bacterial proteins! And...in this new study... this team successfully detects and quantifies pneuomococcal proteins in children with bacterial meningitis. A good bit of this study is optimizing a method for collection, digestion and prep for mass spec. They use in-solution digestion with a commercial MS-compatible detergent prep, and they use 2 hour gradients on 15cm nanoLC columns with an LTQ Orbitrap Velos system. And...loads of bacterial proteins even in the complexity of the CSF proteome! Im no medical expert, but those sound like some seriously sick kids. Progenesis appears to be used for the quantification and all IDs were obtained with Mascot after using the Proteome Discoverer Viewer to create the MGFs. The paper is straight-forward, but the downstream plots for analys...

Proteomics of white button mushrooms post harvest I honestly started reading this paper  because I was 12% awake and the main question I had was something like... If Id stopped at the abstract, Id have known that this group is interested in finding biomarkers that will enable them to select for mushrooms that have enhanced shelf life! As my espresso is being absorbed, this seems like a better idea. I imagine as someone is selectively breeding mushrooms they are probably first focusing on obvious phenotypes like size and shape, but how long that mushroom will last on the shelf might be a lot more problematic to test for at the farm. Off topic: Did you know that portabello and white button mushrooms are all the same species? Just different stages in the maturation process? 5.1 million fungi species on this planet, and in my country we essentially eat just one of them...maybe this is where I should put the Catbug picture I used above.... Back to the paper!  They get a bunch of mu...