The developmental proteome of Drosophila over 15 life stages! Drosophila melanogaster is the geneticists tool! What is someone doing stealing it and doing protein work on it?!? Oh, you know, just finding out more about it than we ever knew! You can check it out in this new paper in GENOME RESEARCH (yeah!) here! They sampled fruit flies at 15 stages of their development -- including early stages that have nauseating names like "pupae". They harvested this grossness and used 2 different protein extraction techniques. Im just a little unclear on this, but it appears that the early embryonic stage organisms need to have the proteins extracted in a different way. Dechlorination is involved. The proteins were separated in the first dimension with SDS-PAGE and peptides digested out for 280 min nanoLC runs into a quadrupole Orbitrap (plus) system running a standard Top15 method. The resulting output? 8 MILLION high resolution MS/MS spectra. The data was all processed in MaxQuant usi...