NYEPLAK

ProteoSign Powerful easy statistics for Proteome Discover and MaxQuant! Have you looked at your output report from Proteome Discoverer -- or even MaxQuant and said something like "Wow, it would be awesome if I could easily get some super advanced statistics on all this quan without having to work very hard?" If so -- Ive got GREAT news for you -- and its called ProteoSign! You can check it out in this nice open paper here. These authors want to supply you with great differential statistics in a fast, simple and free web interface. They set up a nice little server online somewhere that you can access directly here. At this point, ProteoSign appears set up for supporting PD 1.4 and a couple versions of MaxQuant only -- but -- since it is taking the text file output from PD -- I think that it would be able to take data from the new versions as well -- heck, I think if you matched the formatting ProteoSign requires you could put in data from any proteomic software with quantifica...

The developmental proteome of Drosophila over 15 life stages! Drosophila melanogaster is the geneticists tool! What is someone doing stealing it and doing protein work on it?!?  Oh, you know, just finding out more about it than we ever knew! You can check it out in this new paper in GENOME RESEARCH (yeah!) here! They sampled fruit flies at 15 stages of their development -- including early stages that have nauseating names like "pupae". They harvested this grossness and used 2 different protein extraction techniques. Im just a little unclear on this, but it appears that the early embryonic stage organisms need to have the proteins extracted in a different way. Dechlorination is involved. The proteins were separated in the first dimension with SDS-PAGE and peptides digested out for 280 min nanoLC runs into a quadrupole Orbitrap (plus) system running a standard Top15 method. The resulting output? 8 MILLION high resolution MS/MS spectra. The data was all processed in MaxQuant usi...

Proteome Discoverer 2 2 is now available on the Thermo Omics Portal! An amazing scientist at the NIH contacted me and told me that Proteome Discoverer 2.2 is live on the Thermo Omics portal! (Thank you Dr. J!) Demo versions are available, as well as an upgrade key that will work if your copy of Proteome Discoverer has valid maintenance. The instructions to upgrade to PD 2.2 are about the same as the instructions I posted on how to upgrade to PD 2.0 here a while back. Once you get upgraded youll find that PD 2.2 is VERY similar to PD 2.1, just with some awesome new features. For the biggest changes, youll find some videos over there ---> that may be useful (Thank you Dr. P!) download  file  now

The Cell Atlas launches officially today! A subcellular map of the human proteome!! The Cell Atlas launches today!!  12,000 proteins mapped to 30 organelles! Ive gotta run. Check out this blog post at the Human Protein Atlas, it is better than anything Id write anyway!! (and dont skip their cool video if you have time).  Worried about the quality of a protein localization score because they use antibodies to help with this localization? Dont worry, theyve integrated a metric into the quality of their localization score. Antibodies arent perfect, but having a relative scale of the quality of performance sure wont hurt!! Paper in Science today! Was this the best week for proteomics in history, or have I just had too much espresso? download file now