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Quantitative multiplexed protocol for biomarker discovery

Quantitative multiplexed protocol for biomarker discovery


EDIT (5 min after posting and remembering I forgot something that seemed somewhat important...): The subtitle of this paper should be --
HOW TO RELIABLY QUANTIFY 4,000 PROTEINS IN HUMAN PLASMA!


I dont have time this morning to read this whole new Nature Protocols paper as its quite large (19 pages) and Im going to argue that its really several protocols -- but it looks like a seriously valuable resource to me!



What I can tell you:
1) Its exceptionally well-written and clear -- and thorough
2) It shows outstanding reproducibility with isobaric tags -- despite the use of abundant protein depletion (which I generally consider to be something that negatively impacts reproducibility)
3)The troubleshooting section is AWESOME. I suspect at least one of these authors has trained people on these protocols in their facility a few times.

I stored this away to refer to later -- and to send to anyone thinking about doing reporter ion quan based biomarker studies!

download file now

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